Designing Protein-Protein Interactions | a 2025 Rosetta Workshop event

Elevate your skills in protein-protein interaction design and gain insights into the Nobel Prize winning AI & Biophysics-based tools shaping the future of protein engineering.

Cambridge Boston, February 24th -26th 2025

Quick Links

Participant Travel Guide (PPI Workshop 2025)

Rosetta Trainee ONLY Travel Guide (PPI Workshop 2025)

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Workshop Registration (complete before 2/7) https://www.nunify.com/events/wrcworkshop

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Conference Registration (complete before 1/29)

https://winterrosettacon.org/registration/

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<aside> <img src="https://prod-files-secure.s3.us-west-2.amazonaws.com/7dc710dc-3328-4084-99fb-9b223fc0b003/e61dffbe-3c92-44ee-bc9e-8ad142756620/Slack_icon_2019.svg.png" alt="https://prod-files-secure.s3.us-west-2.amazonaws.com/7dc710dc-3328-4084-99fb-9b223fc0b003/e61dffbe-3c92-44ee-bc9e-8ad142756620/Slack_icon_2019.svg.png" width="40px" /> Rosetta Slack Channel: 2025_ppi_workshop

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Slides & Activities

https://github.com/amritan1707/ppi_workshop

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Course Description

Join us for an intensive, hands-on 2.5-day bootcamp designed for industry professionals to master the art of protein-protein interaction (PPI) design. This workshop will cover two distinct and complementary approaches: binder design (small-scale PPIs) and cage design (homo-oligomeric assemblies), providing a comprehensive exploration of the spectrum of PPI applications. Participants will learn to navigate the now "classic" protein design workflow—Diffusion for backbone generation, MPNN for sequence design, Alphafold for structure prediction, and Rosetta for final scoring and analysis—tailored to each application. These methods will showcase major variations in workflows, guiding participants in workflow design decisions, and offering practical strategies for tackling different design challenges.

Key features of the workshop include:

Day 1: Binder Design – Focused on small, precise PPIs.
Day 2: Cage Design – Exploring larger, more complex homo-oligomeric assemblies.
Hands-On Team Project – Apply your learning immediately by working collaboratively to test the tools' capabilities and limitations.

This bootcamp is an intensive learning experience, with long hours, homework, and opportunities to delve deeply into protein design. The sessions will be recorded and shared on YouTube to benefit the broader Rosetta and protein design communities.